The macrolide roxithromycin impairs NADPH oxidase activation and alters translocation of its cytosolic components to the neutrophil membrane in vitro.

Translocation of Its Cytosolic Components NADPH Oxidase Activation and Alters The Macrolide Roxithromycin Impairs

Activation of NADPH oxidase of human neutrophils involves the phosphorylation and the translocation of cytosolic p67phox.

Activation of human neutrophil NADPH oxidase requires the interaction of cytosolic and membrane-associated components. Evidence has been accumulated that in phorbol 12-myristate 13-acetate (PMA)-stimulated neutrophils, the translocation to the plasma membrane of the cytosolic components p47phox and p67phox and the phosphorylation of p47phox are essential steps in activation of NADPH oxidase. No...

Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation.

The reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is part of the microbicidal arsenal used by human polymorphonuclear neutrophils (PMNs) to eradicate invading pathogens. The production of a superoxide anion (O2-) into the phagolysosome is the precursor for the generation of more potent products, such as hydrogen peroxide and hypochlorite. However, this production of O2- is...

Two cytosolic components of the human neutrophil respiratory burst oxidase translocate to the plasma membrane during cell activation.

The superoxide-forming respiratory burst oxidase of human neutrophils is composed of membrane-associated catalytic components and cytosolic constituents required for oxidase activation. This study concerns the hypothesis that cytosolic oxidase components translocate to a membrane fraction when neutrophils are stimulated and the oxidase is activated. A polyclonal antiserum that recognizes two di...

Cytosolic phospholipase A2 regulation of human monocyte NADPH oxidase activity: cPLA2 affects translocation but not phosphorylation of p67phox and p47phox*

The NADPH oxidase of human monocytes is activated, upon exposure to opsonized zymosan and a variety of other stimuli, to catalyze the formation of superoxide anion. Assembly of the NADPH oxidase complex is believed to be a highly regulated process and molecular mechanisms responsible for this regulation remain to be fully elucidated. We have previously reported that cytosolic phospholipase A2 (...